Lucas Mongiat

GROUP LEADER NAME (as it appears in publications):

Lucas A Mongiat

AFFILIATION AND CURRENT POSITION:

Departamento de Fisica Médica

Centro Atómico Bariloche

TEL:* +54 0294 444 5100 int:4852

EMAIL:* lucas.mongiat@cab.cnea.gov.ar

WEB: https://fisica.cab.cnea.gov.ar/fisicamedica/labs-bs/adult-neurogenesis/

GROUP NAME / RESEARCH INTEREST IN FEW WORDS: Adult Neurogenesis

Our goal is to understand the interplay between neural network rearrangement by adult neurogenesis, information processing and cognitive function in the pallium of zebrafish.

SHORT SUMMARY OF RESEARCH INTEREST (MAX. 200 WORDS):

New neurons are produced in large numbers by neuronal progenitor cells distributed along the periventricular zone in the pallium of adult zebrafish. Thus the pallium is subjected to extraordinary plasticity driven by adult neurogenesis. In vertebrates, this structure is involved in complex sensory processing and cognitive operations. My laboratory focuses on studying how different cognitive tasks shape the neural circuits in the zebrafish pallium, as well as on understanding the role that these new neurons exert in orchestrating these behaviors. To approach these questions, we combine transgenic reporter zebrafish lines, immunofluorescence, electrophysiology recordings and behavioral studies. The primal questions leading the focus for current and future research in my laboratory are: To what extent this neuronal circuit dynamic allows reliable information processing and storage leading to adaptive behaviors? How neuronal additions modify the input/output processing of existing networks? Is the cognitive activity a drive for pallial network rearrangements? Could functional regeneration be accomplished to restore lost cognitive ability?

LIST OF UP TO FIVE RELEVANT PUBLICATIONS:

1- Unique Processing During a Period of High Excitation/Inhibition Balance in Adult-Born Neurons. Marin-Burgin A*,Mongiat LA*, Pardi MB and Schinder AF.

Science, 2012;335(6073):1238-42. (*equally contributed)

2- Delayed Coupling to Feedback Inhibition during a Critical Period for the Integration of Adult-Born Granule Cells. SG Temprana*, LA Mongiat*, SM Yang, MF Trinchero, DD Alvarez, E Kropff, D Giacomini, N Beltramone, GM Lanuza and AF Schinder.

Neuron, 2015; 85:116-130. (*equally contributed)

3- Social isolation impairs active avoidance performance and decreases neurogenesis in the dorsomedial telencephalon of rainbow trout. Ausas MS, Mazzitelli-Fuentes L, Roman FR, Crichigno SA, De Vincenti AP, Mongiat LA.

Physiology and Behavior, 2019; 198:1-10.

4- Adult neurogenesis and the plasticity of the dentate gyrus network. Mongiat LA and Schinder AF.

European Journal of Neuroscience, 2011; 33(6):1055-61.

5- Reliable Activation of Immature Neurons in the Adult Hippocampus. Mongiat LA, Esposito MS, Lombardi MG, Schinder AF.

PLoS One, 2009; 4(4): e5320.

GROUP MEMBERS (NAME, POSITION, EMAIL):*

1- Ausas MS, PhD student – maria.ausas@cab.cnea.gov.ar

2- Laura S Mazzitelli-Fuentes, PhD student – laura.mazzitelli@cab.cnea.gov.ar

3- Fernanda R Roman, PhD student – fernanda.r.roman@gmail.com

4- Emilia Deleglise, PhD student – emilia.deleglise@cab.cnea.gov.ar

FISH FACILITIES (TYPE OF FISH SYSTEM/TANKS, CAPACITY, ETC.)*

Facility equiped with a ZS560 rack (Aquaneering Inc, USA). 60 tanks capacity

Watter supply by RO system

FISH LINES KEPT IN STOCK (list according to the following categories: wild type, mutant, transgenic reporter, transgenic knock-in, knock-outs, other. Provide a short description or reference)

1) zebrafish AB - source IBR-CONICET

2) zebrafish Tg(GAD:GFP) - source Shin-Ichi Higashima Lab

3) zebrafish Tg(NeuroD-GCamp6) – source Claire Wyart Lab

4) zebrafish Tg(ubi:lox-GFP-lox-dsRed) – source Laure Bay-Lecuiff Lab

5) zebrafish Tg(her4.1-ERT2.CRE.ERT2)– source Laure Bay-Lecuiff Lab

OTHER EQUIPMENT RELATED TO ZEBRAFISH RESEARCH*

Histology facility, electrophysiology setup, two-photon microscope, molecular biology facility

LAB EXPERTISE AND TECHNICAL CAPABILITIES (RELATED TO ZEBRAFISH RESEARCH)*

Histology, behavioural procedures (spatial tasks, avoidance conditioning, novel tank test), ex vivo electrophysiological recordings (field recordings and patch clamp), ex-vivo calcium imaging

OTHER RELEVANT INFORMATION:*