Niels Olsen Saraiva Câmara

GROUP LEADER NAME (as it appears in publications): Niels Olsen Saraiva Câmara

AFFILIATION:

Full Professor of Immunology. Laboratory for Transplantation Immunobiology

Department of Immunology. Institute of Biomedical Sciences. University of Sao Paulo.

University of Sao Paulo. Av Prof. Lineu Prestes 1730, 05508-000. Cidade Universitária, Sao Paulo, Brazil.

TEL:* (55 11) 3091 7388

FAX:* (55 111) 3091 7224

EMAIL:* niels@icb.usp.br; niels.camara@gmail.com

WEB: http://www.icb.usp.br/~imunoicb/

GROUP NAME / RESEARCH INTEREST IN FEW WORDS:

Laboratory of Transplantation Immunobiology.

Our lab has devoted the past years to study the inflammatory response in non-pathogen injuries. Mainly, we developed several animal models to study the role of innate and adaptive immune responses. Moreover, we were interested in investigating the cytoprotection induced by heme oxygenase and by stem cells.

SHORT SUMMARY OF RESEARCH INTEREST (MAX. 200 WORDS):

In recent years, several research studies have consistently shown the role of the inflammatory response in some kidney diseases. Most prominent data strongly indicate that acute and chronic renal injuries are influenced by the immune response, directed against various compartments of the nephron. Now considered an epidemic, renal diseases have enormous social and economic impact with high morbidity and mortality for patients. Thus, the acquisition of new knowledge that will strengthen the knowledge on the pathogenesis of these diseases, and the discovery of new strategies to improve or even reverse the progression of the disease might become necessary to improve patient survival. In our research area, we intend to deepen the studies on the involvement of the inflammatory response in renal lesions, in the current context, taking into account new molecules, lymphocyte subsets and molecules with new functions in different models of acute and chronic injuries.

LIST OF UP TO FIVE RELEVANT PUBLICATIONS:

1- Moraes-Vieira PM, Larocca RA, Bassi EJ, Peron JP, Andrade-Oliveira V, Wasinski F, Araujo R, Thornley T, Quintana FJ, Basso AS, Strom TB, Câmara NO. Leptin deficiency impairs maturation of dendritic cells and enhances induction of regulatory T and Th17 cells. Eur J Immunol. 2013 Nov 22. doi: 10.1002/eji.201343592

2- Correa-Costa M, Azevedo H, Amano MT, Gonçalves GM, Hyane MI, Cenedeze MA, Renesto PG, Pacheco-Silva A, Moreira-Filho CA, Câmara NO. Transcriptome analysis of renal ischemia/reperfusion injury and its modulation by ischemic pre-conditioning or hemin treatment. PLoS One. 2012;7(11):e49569. doi: 10.1371/journal.pone.0049569.

3- Moraes-Vieira PM, Bassi EJ, Larocca RA, Castoldi A, Burghos M, Lepique AP, Quintana FJ, Araujo RC, Basso AS, Strom TB, Câmara NO. Leptin deficiency modulates allograft survival by favoring a Th2 and a regulatory immune profile. Am J Transplant. 2013 Jan;13(1):36-44. doi: 10.1111/j.1600-6143.2012.04283.x.

4 - Braga TT, Correa-Costa M, Guise YF, Castoldi A, de Oliveira CD, Hyane MI, Cenedeze MA, Teixeira SA, Muscara MN, Perez KR, Cuccovia IM, Pacheco-Silva A, Gonçalves GM, Camara NO. MyD88 signaling pathway is involved in renal fibrosis by favoring a TH2 immune response and activating alternative M2 macrophages. Mol Med. 2012 Oct 24;18:1231-9. doi: 10.2119/molmed.2012.00131.

5 - Bassi ÊJ, Moraes-Vieira PM, Moreira-Sá CS, Almeida DC, Vieira LM, Cunha CS, Hiyane MI, Basso AS, Pacheco-Silva A, Câmara NO. Immune regulatory properties of allogeneic adipose-derived mesenchymal stem cells in the treatment of experimental autoimmune diabetes. Diabetes. 2012 Oct;61(10):2534-45. Epub 2012 Jun 11.

GROUP MEMBERS (NAME, POSITION, EMAIL):*

1- Camila Morales, MsC Student, ci.moralesfe@gmail.com

2- Felipe Grabarz, MsC Student, fg.biomed@gmail.com

3- Cristhiane Favero Aguiar, PhD Student, cris_cfa@yahoo.com.br

4- Marina Burgos da Silva, PhD Student, mburgos.silva@gmail.com

5- Angela Castoldi, PhD Student, angela.castoldi@gmail.com

6- Vinicius Andrade de Oliveira, PhD Student, andradevinicius1@gmail.com

7- Daniel May de Oliveira, PhD Student, daniel_may@superig.com.br

8- Raphael José Felizardo, PhD Student, rapha.felizardo@gmail.com

9 - Andrea Caricilli, Post Doc, caricilli@gmail.com

10 – Mariane Tami Amano, Post Doc, mary_amano@yahoo.com

11 – Felipe Valença, Post Doc, felipevalencap@yahoo.com.br

12- Danilo Candido Almeida, Post Doc, gudaalmeida@gmail.com

13 – Clarice Origassa, Post Doc, clarice.jp@gmail.com

14 – Debora Tavares Abate, Post Doc, deboratavares.silva@hotmail.com

15 – Milton Moraes, Post Doc, mrmoraes@unifesp.br

16 – Meire Hiyane, Technician, miy@icb.usp.br

17 – Claudia Silva Cunha, Technician, clasc@usp.br

FISH FACILITIES (TYPE OF FISH SYSTEM/TANKS, CAPACITY, ETC.)*

The animals stay in a stand of the brand Zebtec, developed exclusively for zebrafish maintenance. The fish tanks are of 3.5 L and 8 L, all with a cover. The small tanks can sustain 5 to 10 fishes and the big ones 15 to 20 fishes. The system keep a temperature of 28 °C, pH 7-7.5, and a conductivity of 510-525. Furthermore, the water that supplies the tanks comes from a system of Millipore filters. The room is maintained to a temperature of 22 °C and a timer turns on the lights at 9 am and turn off the lights at 23 pm.

FISH LINES KEPT IN STOCK:*

We have an undefined wild-type line in stock.

OTHER EQUIPMENT RELATED TO ZEBRAFISH RESEARCH*

LAB EXPERTISE AND TECHNICAL CAPABILITIES (RELATED TO ZEBRAFISH RESEARCH)*

Camila Morales, MsC Student, has an experience of 2 years working with the model in the laboratory of the Dra. Alicia Colombo in the University of Chile. There, she learns the basic care of daily maintenance of adult fishes, like preparation of the reverse osmosis water and artemia, alimentation and breeding of the fishes. In her work with the zebrafish embryo, she manipulates the embryo daily and uses techniques like in situ hybridization, immunofluorescence and electroporation of the zebrafish brain. Nowadays she applies techniques like extraction of RNA for RT-PCR and qPCR, histology and immunohistochemistry with zebrafish embryos.

OTHER RELEVANT INFORMATION:*

Transplant Immunobiology Laboratory (LIT) was created in August 2005 within the Department of Immunology, Institute of Biomedical Sciences IV, USP. LIT has as main objective to study the immunological factors that adversely affect the survival of patients receiving transplanted organs. Within this context, a team of researchers and collaborators studied, from the immunological point of view, different diseases, their pathophysiological mechanisms and new therapeutic approaches that can improve the outcomes of these injuries.

In the zebrafish field, our group is working altogether with the Special Laboratory of Applied Toxicology, in the Butantan Institute that provides the zebrafish facilities for the development of our projects.

SUMMARY OF RESEARCH INTEREST (MAX. 2000 WORDS)

Research Area: Regulation of Inflammatory Response in Experimental Renal Injuries

Head of research: Prof Niels Olsen Saraiva Camara

In recent years, several research groups nationally and internationally, including ours, have consistently shown the role of the inflammatory response in some kidney diseases. Despite advances most prominent data have been generated in animal models, they strongly indicate that acute and chronic renal injuries are influenced by the immune response, directed against various compartments of the nephron. Now considered an epidemic, renal diseases have enormous social and economic impact with high morbidity and mortality for patients. Thus, the acquisition of new knowledge that will strengthen the knowledge on the pathogenesis of these diseases, and the discovery of new strategies to improve or even reverse the progression of the disease might become necessary to improve patient survival. Specifically, the results obtained by our group over the years show that both elements of the innate immune response, such as activation of endothelial cells, the action of cyclooxygenase and bradykinin, as well as elements of the adaptive immune response, such as CD4 + T lymphocytes participate as effectors of renal injury. Simultaneously, we have also seen that renal tissue is able to mount a response cytoprotective ranging from reorganization of the expression of proteins in the endoplasmic reticulum to the action of HO- 1. More recently, new molecules, hormones and cell subtypes have been identified and shown to participate in various stages of the inflammatory response. With the changing lifestyle, new factors have been added to the list of co-morbidities of renal diseases, such as obesity. Along with this, new adipogenicity-related hormones, changes in intestinal microflora and inflammatory molecules have been given an important role in the pathogenesis of renal injury. In our research area, we intend to deepen the studies on the involvement of the inflammatory response in renal lesions, in the current context, taking into account new molecules, lymphocyte subsets and molecules with new functions in different models of acute and chronic injuries. We intend to answer the hypothesis and objectives.