Esteves
GROUP LEADER NAME (as it appears in publications):
Adriana Esteves
AFFILIATION:
TEL:* 2 525 2095
FAX:* 2 525 8617
EMAIL:*aesteves@gmail.com
WEB:
GROUP NAME / RESEARCH INTEREST IN FEW WORDS:
We are interested in the study of enterocyte lipid absorption.
SHORT SUMMARY OF RESEARCH INTEREST (MAX. 200 WORDS):
Note: please attach at the end of the form a longer description, with a maximum of 2000 words.
In the Western diet, lipids represent more than 40% of the daily caloric intake, while the nutritional advice is 10% lower. This high fat supply associated with a qualitative imbalance greatly contributes not only to the increase of obesity prevalence in the population, but also to the appearance of diseases such as atheroclerosis, non insulin-dependent diabetes, breast and colon cancers. Although the gut is responsible for fat absorption, little is known about the mechanisms involved in the uptake and metabolic fate of lipids or their metabolic derivatives in the intestinal cell.
The mechanisms underlying the uptake and transport of extra cellular FA are incompletely known. Membrane-associated proteins may play a crucial role in the release of FA from the inner leaflet of the plasma membrane into the cytoplasm by a collisional interaction with FABPs or into the water layer adjacent to the membrane prior to binding to FABPs.
Three different FABPs are expressed in the intestine: I-FABP, L-FABP, and il-FABP. I-FABP has a specific localization along the small intestine, suggesting a cellular specialization.
The role of these FABPs in the uptake of FA from diet is been studied by our group using zebra fish as a model.
LIST OF UP TO FIVE RELEVANT PUBLICATIONS:
We have no publication on Zebra fish.
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GROUP MEMBERS (NAME, POSITION, EMAIL):*
1- Adriana Esteves, Full time Associate Professor; aesteves@fcien.edu.uy
2- Mariel Flores, pregraduate student; flores2101@hotmail.com
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FISH FACILITIES (TYPE OF FISH SYSTEM/TANKS, CAPACITY, ETC.)*
Our group has taken recently zebrafish as a model. We initiate our work in collaboration with Dr. Patrick Babin from Bordeaux 1 University, and used his facilities for these first steps.
FISH LINES KEPT IN STOCK:*
OTHER EQUIPMENT RELATED TO ZEBRAFISH RESEARCH*
Molecular biology facilities: micro and ultra centrifugues, electrophoresis equipment, chromatographic equipment, spectrofotometer, -80°C and -20°C freezers, stoves, thermostated shakers, PCR machines. Accessible services: transmision electron microscope, confocal microsocope, sequencing, qRT-PCR.
LAB EXPERTISE AND TECHNICAL CAPABILITIES (RELATED TO ZEBRAFISH RESEARCH)*
Molecular biology inmunohistochemical and techniques
OTHER RELEVANT INFORMATION:*