Danillo Pinhal

GROUP LEADER NAME (as it appears in publications):

Danillo Pinhal

link lattes: http://lattes.cnpq.br/4860817175728682

link google scholar: http://scholar.google.com/citations?user=sUqB6bUAAAAJ

link researcher ID: http://www.researcherid.com/rid/C-5983-2012

AFFILIATION: Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP

 TEL:* +55 14 3880 0381

 FAX:*

 EMAIL:* dlpinhal@ibb.unesp.br ; dlpinhal@gmail.com

 WEB: http://ibb.unesp.br/#!/departamentos/genetica/

GROUP NAME / RESEARCH INTEREST IN FEW WORDS:

LGEA - Laboratório de Genética e Evolução Animal / Investigate the role of non-coding RNA in shaping animal genome evolution and function; animal conservation genetics

SHORT SUMMARY OF RESEARCH INTEREST (MAX. 200 WORDS):

We aim to uncover the role played by small noncoding RNAs during vertebrate development, focusing mainly on microRNAs. We also seek to understand the evolutionary dynamics of microRNAs using fish (e.g., zebrafish and Nile Tilapia) as model and/or target organisms.

LIST OF UP TO FIVE RELEVANT PUBLICATIONS:

1- Martins C, Cabral-de-Mello DC, Valente GT, Mazzuchelli J, Oliveira SG, Pinhal D (2011). Animal genomes under the focus of cytogenetics. Nova Science Publisher, Hauppauge, NY, USA. 160 pp.

2- Pinhal, D.; Shivji, Mahmood S.; Nachtigall, Pedro G.; Chapman, Demian D.; Martins C. A Streamlined DNA Tool for Global Identification of Heavily Exploited Coastal Shark Species (Genus Rhizoprionodon). Plos One, v. 7, p. e34797, 2012.

3- Pinhal, D.; Yoshimura, Tatiana S ; Araki, Carlos S ; Martins C. The 5S rDNA family evolves through concerted and birth-and-death evolution in fish genomes: an example from freshwater stingrays. BMC Evolutionary Biology (Online), v. 11, p. 151, 2011.

4- Pinhal, D.; Shivji, M. S. ; Vallinoto, M. ; Chapman, D. D. ; Gadig, O.B.F. ; Martins, C. Cryptic hammerhead shark lineage occurrence in the western South Atlantic revealed by DNA analysis. Marine Biology (Berlin), v. 159, p. 829-836, 2012.      

GROUP MEMBERS (NAME, POSITION, EMAIL):*

1- Pedro Gabriel Nachtigall - PhD student, pedronachtigall@yahoo.com

2- Luiz Augusto Bovolenta - PhD student (co-advising), labovolenta@gmail.com

3- Helder Elias da Silva - Master student, helder.eliasilva@gmail.com   

4- Arthur Casulli de Oliveira - Undergraduate student, arthur.biologia.unesp@gmail.com

5- Camila Lovaglio Campos - Undergraduate student, camilalovaglio@gmail.com

FISH FACILITIES (TYPE OF FISH SYSTEM/TANKS, CAPACITY, ETC.)*

We are building a small facility for zebrafish and we will keep them in the ZebTec Stand Alone System (Tecniplast).

Cooperation with Prof. Cesar Martins gives access to his cichlid fish facility.

FISH LINES KEPT IN STOCK:*

We don't have any zebrafish lines in stock.

OTHER EQUIPMENT RELATED TO ZEBRAFISH RESEARCH*.

We are buying a stereomicroscope prepared with fluorescence and camera, a computer coupled with image analysis system, microinjection system and a PCR machine with temperature gradient.

We already have a Real time PCR system (StepOne Plus - Life Technologies) and a high performance sequencer (MiSeq - Illumina), as well as other basic apparatus for molecular biology experiments.

LAB EXPERTISE AND TECHNICAL CAPABILITIES (RELATED TO ZEBRAFISH RESEARCH)*

We are beginners in running experiments with zebrafish. Actually we had never worked with zebrafish in my lab before. Our previous experiences were handling Nile tilapia and Elasmobranchii (sharks and rays).

I did a short training with zebrafish at Vanderbilt University, EUA, in the lab of professor James Patton a few years ago.