Costa

GROUP LEADER NAME (as it appears in publications):

Manoel Luis Costa

AFFILIATION AND CURRENT POSITION:

Universidade Federal do Rio de Janeiro, full professor (“professor titular”)

TEL:* 55-21-39386429

EMAIL:* manoelluiscosta@ufrj.br

WEB: www.histo.ufrj.br/LabMus

GROUP NAME / RESEARCH INTEREST IN FEW WORDS:

Laboratório de Diferenciação Muscular e Citoesqueleto

SHORT SUMMARY OF RESEARCH INTEREST (MAX. 200 WORDS):

We study muscle differentiation, focusing on the role of cytoskeleton, in zebrafish. Through the analysis of structure and function of markers of differentiation, we hope to understand the cell biology of myogenesis. We have been concentrating on the role of cholesterol, membrane rafts and signaling in myogenesis.

LIST OF UP TO FIVE RELEVANT PUBLICATIONS: 

1- Campos LM, Rios EA, Guapyassu L, Midlej V, Atella GC, Herculano-Houzel S,

Benchimol M, Mermelstein C, Costa ML. Alterations in zebrafish development

induced by simvastatin: Comprehensive morphological and physiological study,

focusing on muscle. Exp Biol Med (Maywood). 2016 Nov;241(17):1950-1960. Epub 2016

Jul 20. PubMed PMID: 27444151; PubMed Central PMCID: PMC5068466.

2-Campos LM, Rios EA, Midlej V, Atella GC, Herculano-Houzel S, Benchimol M,

Mermelstein C, Costa ML. Structural analysis of alterations in zebrafish muscle

differentiation induced by simvastatin and their recovery with cholesterol. J

Histochem Cytochem. 2015 Jun;63(6):427-37. doi: 10.1369/0022155415580396. Epub

2015 Mar 18. PubMed PMID: 25786435; PubMed Central PMCID: PMC4872197.

3-Costa ML, Escaleira RC, Jazenko F, Mermelstein CS. Cell adhesion in zebrafish

myogenesis: distribution of intermediate filaments, microfilaments, intracellular

adhesion structures and extracellular matrix. Cell Motil Cytoskeleton. 2008

Oct;65(10):801-15. doi: 10.1002/cm.20301. PubMed PMID: 18680203.

4-Costa ML, Escaleira RC, Rodrigues VB, Manasfi M, Mermelstein CS. Some

distinctive features of zebrafish myogenesis based on unexpected distributions of

the muscle cytoskeletal proteins actin, myosin, desmin, alpha-actinin, troponin

and titin. Mech Dev. 2002 Aug;116(1-2):95-104. PubMed PMID: 12128209.

5-Costa ML, Escaleira RC, Rodrigues VB, Manasfi M, Mermelstein CS. Some

distinctive features of zebrafish myogenesis based on unexpected distributions of

the muscle cytoskeletal proteins actin, myosin, desmin, alpha-actinin, troponin

and titin. Mech Dev. 2002 Aug;116(1-2):95-104. PubMed PMID: 12128209.

GROUP MEMBERS (NAME, POSITION, EMAIL):*

1- Manoel Luis Costa, professor, manoelluiscosta@ufrj.br

2- Claudia Mermelstein, professor, mermelstein@ufrj.br

3- Geyse Gomes, graduate student, geyse.goms@gmail.com

4- Cyro Gomes, undergraduate student, cyro.gs@hotmail.com

FISH FACILITIES (TYPE OF FISH SYSTEM/TANKS, CAPACITY, ETC.)*

Homemade fish system, with parts from Aquatic Habitats, with 100 tanks, 1500 fish capacity

FISH LINES KEPT IN STOCK (list according to the following categories: wild type, mutant, transgenic reporter, transgenic knock-in, knock-outs, other. Provide a short description or reference)

wild type

OTHER EQUIPMENT RELATED TO ZEBRAFISH RESEARCH*

Dissecting scope with microinjection, fluorescence microscope and disc confocal microscope.

LAB EXPERTISE AND TECHNICAL CAPABILITIES (RELATED TO ZEBRAFISH RESEARCH)*

Our strongest expertise is on optical microscopy, including confocal and super-resolution microscopy.