Allende

GROUP LEADER NAME (as it appears in publications): Miguel L Allende

AFFILIATION AND CURRENT POSITION:

Full Professor. Facultad de Ciencias, Universidad de Chile

Director. Center for Genome Regulation

TEL:* +56 2 229787351

EMAIL:* mallende@uchile.cl

GROUP NAME / RESEARCH INTEREST IN FEW WORDS: Zebrafish for Innovation and Research Lab.

SHORT SUMMARY OF RESEARCH INTEREST (MAX. 200 WORDS): We are interested in molecular and cellular mechanisms through which the vertebrate organism maintains tissue and organ homeostasis. We use the zebrafish and develop tools aimed at understanding processes of tissue regeneration, immunity and metabolic balance. We also explore the genetic and molecular bases for these phenomena.

LIST OF UP TO FIVE RELEVANT PUBLICATIONS:

Morales RA, Allende ML. 2019. Peripheral Macrophages Promote Tissue Regeneration in Zebrafish by Fine-tuning the Inflammatory Response. Front. Immunol. 10:253. doi: 10.3389/ﬁmmu.2019.00253.

Paredes-Zúñiga S, Trost N, De la Paz J, Alcayaga J, Allende ML. 2019. Behavioral effects of triadimefon in zebrafish are associated with alterations of the dopaminergic and serotonergic pathways. Prog. Neuro-Psychopharmacol. Biol. Psychiatry. 92:118-126. https://doi.org/10.1016/j.pnpbp.2018.12.012

Parada M, Clatworthy A, Hagendorn EJ, Ernst C, Henninger JE, Riquelme R, Jijón H, Villablanca EJ, Zon LI, Hung D, Allende ML. 2018. Generation of Mouse-Zebrafish Hematopoietic Tissue Chimeric Embryos by Xenotransplantation. Dis. Model. Mech. 11: dmm034876. doi: 10.1242/dmm.034876.

Díaz-Pascual F, Ortíz-Severín J, Varas M, Allende ML*, Chávez FP*. 2017. In vivo host-pathogen interaction as revealed by global proteomic profiling of zebrafish larvae. Front. Cell. Infect. Microbiol. 7:334.

Sánchez M, Ceci, ML, Gutiérrez D, Anguita C, Allende ML. 2016. Mechanosensory organ regeneration in zebrafish depends on a population of multipotent progenitor cells kept latent by Schwann cells. BMC Biology 14: 27.

GROUP MEMBERS (NAME, POSITION, EMAIL):*

An up to date list can be found at: http://zebrafish.cl/current-lab-members.html

FISH FACILITIES (TYPE OF FISH SYSTEM/TANKS, CAPACITY, ETC.)*

One multilinking system (6 racks) by Tecniplast

Five stand alone systems (Tecniplast)

One home made glass acquaria system

Total of approximately 450 tanks for zebrafish in two dedicated facilities (one is quarantined)

A separate fish room for annual killifish (24 tanks)

FISH LINES KEPT IN STOCK (list according to the following categories: wild type, mutant, transgenic reporter, transgenic knock-in, knock-outs, other. Provide a short description or reference)

Too many to list; can provide a link with our database

OTHER EQUIPMENT RELATED TO ZEBRAFISH RESEARCH*

A home made light sheet microscope, red and green fluorescence channels. A two-photon microscope. Behavioral tracking device for larvae. Small screening facility for chemicals, molecules, contaminants, pharmaceuticals.

LAB EXPERTISE AND TECHNICAL CAPABILITIES (RELATED TO ZEBRAFISH RESEARCH)*

Experience with morphological, physiological and behavioral screens, using larvae. Also, testing of food additives in adults for the fisheries and aquaculture industries.

Oxford Nanopore sequencing capabilities. CRISPR/Cas9 mutagenesis capability.

OTHER RELEVANT INFORMATION:*

Relevant experience in educational tools using zebrafish: portable labs for high schools, secondary school teacher training, stands for science fairs.